Potatoes with reduced granule-bound starch synthase
AssigneeCellectis
InventorsBenjamin Clasen, Daniel F. Voytas, Feng Zhang
Life of this patent
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Abstract
Materials and methods for making plants (e.g., Solanum varieties) with decreased levels of amylose are provided herein. The methods can include making mutations in the gene encoding granule bound starch synthase (GBSS), where the mutations are induced using a rare-cutting endonuclease.
Claims40
A Solanum plant, plant part, or plant cell comprising a mutation in at least three granule-bound starch synthase (GBSS) allele endogenous to said plant, plant part, or plant cell, such that said plant, plant part, or plant cell has reduced expression of GBSS and decreased levels of amylose as compared to a control Solanum plant, plant part, or plant cell that lacks said mutation.
A method for making a Solanum plant, comprising:
- (a) contacting a population of Solanum plant cells comprising a functional GBSS allele with a rare-cutting endonuclease targeted to an endogenous GBSS sequence,
- (b) selecting, from said population, a cell in which at least three GBSS alleles have been inactivated, and
- (c) growing said selected plant cell into a Solanum plant, wherein said Solanum plant has reduced levels of amylose as compared to a control Solanum plant in which said at least three GBSS alleles have not been inactivated.
A method for producing a starch product, comprising:
- (a) providing a Solanum plant or plant part that comprises a mutation in at least three GBSS alleles endogenous to said plant or plant part, such that said plant, plant part, or plant cell has reduced expression of granule-bound starch synthase and decreased levels of amylose as compared to a control Solanum plant or plant part that lacks said mutation;
- and
- (b) producing a starch product from said plant or plant part.
A starch product produced from a Solanum plant or plant part that comprises a mutation in each GBSS allele endogenous to said plant or plant part, such that said plant, plant part, or plant cell has no functional GBSS allele, wherein said starch product has reduced levels of amylose as compared to a starch product made from a control plant or plant part that lacks said mutation.
Dependencies are inferred from the claim text.