Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms
AssigneeOrganobalance GmbH
InventorsChristine Lang, Markus Veen
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Abstract
The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
Claims60
A method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms which, compared to the wild type, have an increased activity of at least one of the activities selected from the group consisting of Δ8-Δ7-isomerase activity, Δ5-desaturase activity and Δ24-reductase activity.
A nucleic acid construct, comprising at least one nucleic acid selected from the group consisting of nucleic acids encoding a Δ8-Δ7-isomerase, nucleic acids encoding a Δ5-desaturase and nucleic acids encoding a Δ24-reductase, which are functionally linked with one or more regulatory signals ensuring transcription and translation in organisms.
A combination of nucleic acid constructs, which comprises at least one nucleic acid construct selected from the groups A to C A nucleic acid construct comprising nucleic acids encoding a Δ8-Δ7-isomerase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, B nucleic acid construct comprising nucleic acids encoding a Δ5-desaturase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms and C nucleic acid construct comprising nucleic acids encoding a Δ24-reductase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, and at least one nucleic acid construct selected from the groups D to H D nucleic acid construct comprising nucleic acids encoding an HMG-CoA reductase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, E nucleic acid construct comprising nucleic acids encoding a lanosterol C14-demethylase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, F nucleic acid construct comprising nucleic acids encoding a squalene epoxidase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, G nucleic acid construct comprising nucleic acids encoding a squalene synthetase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms, H nucleic acid construct comprising nucleic acids encoding a sterol acyltransferase, which are functionally linked to one or more regulatory signals ensuring transcription and translation in organisms.
A genetically modified organism, wherein the genetic modification increases at least one of the activities selected from the group consisting of Δ8-Δ7-isomerase activity, Δ5-desaturase activity and Δ24-reductase activity, compared to a wild type.
Dependencies are inferred from the claim text.