Biological methods for preparing a fatty dicarboxylic acid
AssigneeVerdezyne, Inc.
InventorsTom BEARDSLEE, Alex HUTAGALUNG, Stephen PICATAGGIO
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Abstract
The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.
Claims202
A genetically modified Yarrowia lipoytica yeast comprising (i) an active, modified endogenous acyl-coA oxidase polypeptide or an active, modified endogenous acyl-coA dehydrogenase polypeptide;
- (ii) a genetic modification that alters a native enoyl co-A isomerase activity;
- and (iii) a genetic modification that alters a native dieneoyl CoA reductase (DCR) activity, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.
A genetically modified yeast comprising an active, modified endogenous acyl-CoA oxidase polypeptide or an active, modified endogenous acyl-CoA dehydrogenase polypeptide, which yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.
The genetically modified yeast of claim, wherein the Candida spp. yeast is a genetically modified ATCC20336 yeast.
A genetically modified yeast comprising (i) an active, modified endogenous acyl-CoA oxidase polypeptide or an active, modified endogenous acyl-CoA dehydrogenase polypeptide and (ii) a genetic modification that alters an enoyl CoA isomerase activity, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.
A genetically modified yeast, comprising a heterologous acyl-CoA oxidase polypeptide or a heterologous acyl-CoA dehydrogenase polypeptide, which yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.
An isolated nucleic acid, comprising a polynucleotide that encodes a modified acyl-CoA oxidase polypeptide from a yeast.
A genetically modified yeast comprising (i) an active, modified endogenous acyl-coA oxidase polypeptide or an active, modified endogenous acyl-coA dehydrogenase polypeptide;
- (ii) a genetic modification that alters an enoyl co-A isomerase activity;
- and (iii) a genetic modification that alters a dieneoyl CoA reductase (DCR) activity, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.
A method for producing a diacid, comprising:
- (a) contacting a genetically modified yeast of any of embodiments 1-124 and 160-198 with a feedstock comprising an unsaturated vegetable fatty acid capable of being converted by the yeast to a diacid;
- (b) culturing the yeast under conditions in which diacid containing one or more unsaturations is produced from the feedstock;
- and
- (c) hydrogenating and removing one or more of the unsaturations.
Dependencies are inferred from the claim text.