Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same
AssigneeLG Chem, Ltd.
InventorsSi Jae PARK, Sang Hyun LEE, Sang Yup LEE, Eun Jeong LEE
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Abstract
A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
Claims27
1-32. (canceled)
An isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate, and 4-hydroxybutyrate into 1,4-butanediol, in a Lumen bacteria capable of producing succinate.
A isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying a gene encoding Cat1;
- a gene encoding SucD;
- a gene encoding 4hbD or GHB;
- a gene encoding 4-hydroxybutyrate-CoA transferase, or a gene encoding Ptb and a gene encoding Buk;
- and a gene encoding butyl-CoA dehydrogenase, in a microorganism capable of producing succinate.
Dependencies are inferred from the claim text.