US 2015/0344837 A1 · MINIMAL BACTERIAL GENOME

Abstract

The present invention relates, e.g., to a minimal set of protein-coding genes which provides the information required for replication of a free-living organism in a rich bacterial culture medium, wherein (1) the gene set does not comprise the 100 genes listed in Table 2; and/or wherein (2) the gene set comprises the 382 protein-coding genes listed in Table 3 and, optionally, one of more of: a set of three genes encoding ABC transporters for phosphate import (genes MG410, MG411 and MG412; or genes MG289, MG290 and MG291); the lipoprotein-encoding gene MG185 or MG260; and/or the glycerophosphoryl diester phosphodiesterase gene MG293 or MG385.

Claims16

Dependent claims

A non-naturally occurring free-living prokaryotic organism comprising a plurality of bacterial genes comprised on one or more nucleic acid molecules,

wherein the plurality of bacterial genes encode at least 351 proteins but not more than 450 proteins, and wherein:
(i) the at least 351 proteins are encoded by a minimal gene set and are required for growth and replication of a free-living bacterial organism under axenic conditions in a rich bacterial medium;
and
(ii) the at least 351 proteins perform at least the functions of the genes set forth in Table 3;
and
(iii) further comprising at least one expressible heterologous gene.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/733,743Filed 8 Jun 2015

Priority

12 Oct 2005

Published

3 Dec 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C12N 1/20
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C07K 14/195
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from bacteria
C07K 14/30
From bacteria: from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]
C12N 1/205
Bacteria; Culture media therefor: bacterial isolates

Minimal bacterial genome

Life of this patent

Minimal bacterial genome

Life of this patent

Abstract

Claims16