# US 2015/0338401 A1 · Multiplexed bioassay techniques > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2015/0338401 A1 - Document ID: US20150338401A1 - Status: Published application - Kind: A1 - Application number: 14/443,504 - Filed: 2013-11-18 - Published: 2015-11-26 - Earliest claimed: 2012-11-16 - Assignees: Lightstat, Inc. - Applicants: Lightstat, LLC - Inventors: Ayal Ram - Web page: https://patentgrep.com/patent/US20150338401A1 ## CPC - G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings} - B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip} - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - B01L 2300/0636: Integrated biosensor, microarrays - B01L 2300/0825: Test strips - B01L 2400/0487: fluid pressure, pneumatics - B01L 2400/086: using baffles or other fixed flow obstructions - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract Techniques for multiplexed bioassays include a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port. A first portion of the microchannel is configured to supply multiple different labeled probes, each selected to complex with one of a corresponding plurality of different analytes. A second portion of the microchannel comprises multiple corresponding different supplementary probes covalently bound to the substrate. A supplementary probe is selected to bind to a part of a corresponding analyte, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe. The techniques include a sensor configured to detect signals emitted from the labeled probes in the second portion of the microchannel. ## Claims ### Claim 1 A microfluidic device comprising; a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port, wherein a first portion of the microchannel is configured to supply a plurality of different labeled probes, each selected to complex with one of a corresponding plurality of different analytes; and a second portion of the microchannel comprises a plurality of corresponding different supplementary probes covalently bound to the substrate, wherein a supplementary probe of the plurality of corresponding different supplementary probes is selected to bind to a part of a corresponding analyte of the corresponding plurality of different analytes, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe of the plurality of different labeled probes; and a sensor configured to detect signals emitted from the plurality of labeled probes in the second portion of the microchannel. ### Claim 2 (depends on 1) A device as recited in claim 1, further comprising an actuator configured to move a sample fluid from the entry port to the first portion of the microchannel and then to the second portion of the microchannel and then to the exit port. ### Claim 3 (depends on 1) A device as recited in claim 1, wherein the second portion of the microchannel does not overlap the first portion of the microchannel. ### Claim 4 (depends on 1) A device as recited in claim 1, wherein the first portion of the microchannel further comprises a hydrogel that encompasses the plurality of different labeled probes. ### Claim 5 A method comprising: providing a microfluidic device comprising a sensor and a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port, wherein a first portion of the microchannel is configured to supply a plurality of different labeled probes, each selected to complex with one of a corresponding plurality of different analytes, and a second portion of the microchannel comprises a plurality of corresponding different supplementary probes covalently bound to the substrate, wherein a supplementary probe of the plurality of corresponding different supplementary probes is selected to bind to a part of a corresponding analyte of the corresponding plurality of different analytes, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe of the plurality of different labeled probes, and the sensor is configured to detect signals emitted. from the plurality of labeled probes in the second portion of the microchannel; moving a sample fluid from the entry port to the exit port; and obtaining data from the sensor that indicates measurements of the signals emitted from probes bound to the supplementary probes in the second portion during an observation period. ### Claim 6 (depends on 5) A method as recited in claim 5, further comprising determining a presence of any of the plurality of analytes based on the data obtained from the sensor the sensor. ### Claim 7 (depends on 5) A method as recited in claim 5, further comprising determining at least one of a relative quantity or absolute quantity of any of the plurality of analytes based on the data obtained from the sensor. ### Claim 8 (depends on 5) A method as recited in claim 5, further comprising flushing unbound constituents from the microchannel before obtaining data from the sensor. ### Claim 9 A kit comprising: a device comprising a sensor and a substrate in which is formed a microchannel in fluid communication between an entry port and an exit port, wherein a first portion of the microchannel is configured to supply a plurality of different labeled probes, each selected to complex with one of a corresponding plurality of different analytes, and a second portion of the microchannel configured for covalently binding a plurality of corresponding different supplementary probes to the substrate, wherein a supplementary probe of the plurality of corresponding different supplementary probes is selected to bind to a part of a corresponding analyte of the corresponding plurality of different analytes, which part is exposed when the corresponding analyte is complexed with a corresponding labeled probe of the plurality of different labeled probes; a supply of a plurality of molecules for each of the plurality of different labeled probes. ### Claim 10 (depends on 9) A kit as recited in claim 9, further comprising a supply of a plurality of molecules for each of the plurality of corresponding different supplementary probes. ### Claim 11 (depends on 10) A kit as recited in claim 10, further comprising a reagent for covalently binding the plurality of corresponding different supplementary probes to the substrate in the second portion of the microchannel.