Vaccine prepared utilizing human parainfluenza virus type 2 vector
AssigneesBiocomo Inc., Mie University
InventorsMasayuki FUKUMURA, Junpei OHTSUKA, Tetsuya NOSAKA, Masato TSURUDOME, Mitsuo KAWANO, Ken-ichiro HARA
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Abstract
Disclosed are: a virus vector in which a gene encoding an antigenic polypeptide is integrated in human parainfluenza virus type 2 gene, wherein the antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein; and a method for producing the same. The virus vector of the present invention contains a quantitatively large amount of the antigenic peptide on the virus particle and can efficiently deliver the antigenic polypeptide to a target cell.
Claims20
A virus vector in which a gene encoding an antigenic polypeptide is integrated in a gene of an F protein-defective virus of the family Paramyxoviridae, wherein the antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein or a portion thereof.
(canceled)
A method for producing a virus vector of the family Paramyxoviridae by which an antigenic polypeptide is expressed in the form of a fusion protein with a viral structural protein or a portion thereof, the method comprising the steps of:
- coculturing, with a Vero cell expressing the F gene of the virus of the family Paramyxoviridae or the F gene fused with the antigen, an F gene-defective virus vector of the family Paramyxoviridae in which a gene encoding the fusion protein is integrated in a viral gene;
- and
- isolating virus particles from the culture supernatant.
(canceled)
A Vero cell comprising antigen gene-fused F gene of a virus of the family Paramyxoviridae and expressing a fusion protein between the antigen and the F protein.
Dependencies are inferred from the claim text.