Methods and compositions for analyte detection

A method for detection of one or more analytes in a fluid sample comprising:

• (a) contacting a fluid sample with a system comprising;
• (i) a sample collection implement comprising
• (1) a swab,
• (2) a chamber containing fluid for extracting said swab,
• (3) a plurality of pairs of first and second detection reagents, each pair capable of specifically binding a different target analyte in said fluid sample;
• wherein either said first or second detection reagents further comprises a capture moiety;
• (ii) a test device comprising a substrate defining a lateral flow path comprising a plurality of test regions positioned within said flow path, said test regions each containing an immobilized reagent capable of specifically binding to said capture moiety, wherein each of the test regions comprises a different immobilized reagent capable of binding a different capture moiety;
• and at least one control region disposed in said lateral flow path,
• wherein said control region permits a determination of proper performance of the sensor;
• (b) detecting whether one or more analytes bind to said immobilized capturing reagent, disposed in said test device;
• and
• (c) determining whether one or more analytes are present in said fluid sample, if a signal is detected in at least one of said plurality of test regions.