Recombinant host cell for biosynthetic production
AssigneeRhodia Operations
InventorsOdile Ramaen, Vincent Sauveplane, Rudy Pandjaitan
Life of this patent
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Abstract
A cell may include heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.
Claims20
A cell comprising heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid including any of phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), or phenylalanine/tyrosine ammonia lyase (PAL/TAL), and optionally one or more further enzymes to convert an aromatic amino acid into coumaric acid, wherein the multienzyme complex further comprises enzymes to convert coumaric acid into vanillin or a hydroxybenzaldehyde precursor thereof, including a crotonase.
Dependencies are inferred from the claim text.