Methods and Systems for Fourier Ptychographic Imaging
AssigneeUniversity of Connecticut
InventorsGuoan Zheng, Siyuan Dong
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Abstract
In one aspect, the present disclosure provides a system for Fourier ptychographic microscopy, the system comprising (i) an image capture apparatus including an objective lens, (ii) at least one processor, and (iii) data storage including program instructions stored thereon that when executed by the at least one processor, cause the system to: (a) capture, via the image capture apparatus, a plurality of initial images of an object, wherein each of the plurality of initial images of the object have a first resolution, and (b) process each of the plurality of initial images in Fourier space to generate a final image of the object having a second resolution, wherein the second resolution is greater than the first resolution.
Claims20
A system for Fourier ptychographic microscopy, the system comprising:
- an image capture apparatus including an objective lens;
- at least one processor;
- and
- data storage including program instructions stored thereon that when executed by the at least one processor, cause the system to:
- capture, via the image capture apparatus, a plurality of initial images of an object, wherein each of the plurality of initial images of the object have a first resolution;
- and
- process each of the plurality of initial images in Fourier space to generate a final image of the object having a second resolution, wherein the second resolution is greater than the first resolution.
A method for structured-illumination-based Fourier microscopy, the method comprising:
- capturing, via an objective lens, a plurality of initial images of an object, wherein the plurality of initial images of the object have a first resolution;
- and
- processing each of the plurality of initial images to generate a final image of the object having a second resolution, wherein the second resolution is greater than the first resolution, and wherein the processing comprises:
- (a) generating an initial estimate image for a high-resolution image of the object;
- (b) multiplying the initial estimate image with an illumination pattern to generate a target image of the object;
- (c) transforming the target image to the Fourier domain to generate a Fourier transform of the target image;
- (d) transforming the Fourier transform of the target image to the spatial domain to generate an updated target image;
- (e) replacing the initial estimate image of the object with the updated target image;
- (f) repeating steps (b)-(e) for each of the plurality of initial images of the object;
- and
- (g) iteratively repeating steps (b)-(f) to generate the final image of the object.
A method for spectrum multiplexing in structured-illumination-based Fourier microscopy, the method comprising:
- capturing, via an objective lens, a plurality of initial images of an object, wherein the plurality of initial images of the object have a first resolution;
- and
- processing each of the plurality of initial images to generate a final image of the object having a second resolution, wherein the second resolution is greater than the first resolution, and wherein the processing comprises:
- (a) generating a first initial estimate image for a high-resolution image of the object at a first wavelength;
- (b) generating a second initial estimate image for a high-resolution image of the object at a second wavelength;
- (c) multiplying the first and second initial estimates with an illumination pattern to generate a first target image and a second target image of the object;
- (d) transforming the first target image and the second target image to the Fourier domain to generate a Fourier transform of the first target image and second target image;
- (e) transforming the Fourier transform of the first target image and second target image to the spatial domain to generate an updated first target image and updated second target image;
- (f) replacing the first initial estimate image of the object with the first updated target image and the second initial estimate image of the object with the second updated target image;
- (g) repeating steps (c)-(f) for each of the plurality of initial images of the object;
- and
- (h) iteratively repeating steps (c)-(g) to generate the final image of the object.
Dependencies are inferred from the claim text.