Methods for assessing immunogenicity
AssigneeSanofi Pasteur Limited
InventorsMarie-Danielle Salha, Scott Gallichan, Roger Brookes, Martina Ochs-Onolemhemhen
Life of this patent
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Abstract
This disclosure relates to methods for detecting the potency of a drug preparation and/or detecting antibodies in a host reactive against one or more antigens.
Claims25
A method comprising the steps of:
- a) contacting a drug product comprising one or more antigens with an antibody composition comprising antibodies reactive against at least one of the one or more antigens to produce a test composition;
- b) contacting the test composition with a test cell expressing at least one of the antigens;
- and,
- c) detecting the binding of antibodies to the test cell.
A method for determining vaccine efficacy in a mammal comprising contacting mammalian sera with a test cell expressing at least one cell surface antigen with which the mammal was previously vaccinated;
- and, detecting the binding of antibodies of the sera, if present therein, to the test cell.
A peptide selected from the group consisting of PhtD-262 (GVAVPHGNHYHFIPY (SEQ ID NO:1), PhtD-290 (FYNKAYDLLARIHQD (SEQ ID NO:2), PhtD-348 SADNLYKPSTDTEET (SEQ ID NO:3), PhtD-349 (YKPSTDTEETEEEAE (SEQ ID NO:4), PhtD-360 (ETLTGLKSSLLLGTK (SEQ ID NO:
- 5), PhtE-404 (YIPKSDLSASELAAA (SEQ ID NO:6), and PhtE-440 (AYIVRHGDHFHYIPK (SEQ ID NO:7).
Dependencies are inferred from the claim text.