Method and biomarkers for the detection of dengue hemorrhagic fever
AssigneeBoard of Regents, The University of Texas System
InventorsTadeusz KOCHEL, Josephina Nores GARCIA, Allan BRASIER, Adrian RECINOS, John E. WIKTOROWICZ, Heidi SPRATT, Hyunsu JU
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for detecting, analyzing, and identifying biomolecules used to identifying patient with dengue-like symptom who are at risk of DHF. The inventive method comprises detecting in a sample from a subject dengue infected patient one or more biomarkers selected from the group consisting of IL-10, fibrinogen, C4A, immunoglobulin, tropomyosin, and three isoforms of albumin, and which are used in a predictive MARS model to detect patients with risk of developing DHF.
Claims7
1-20. (canceled)
A sample fractionation method comprising:
- (a) fractionating a sample by size exclusion chromatography, wherein the sample is spiked with a labeled protein of about 200 amino acid residues;
- (b) collecting a (i) protein pool comprising the fractions preceding the end of the labeled protein peak, and (ii) a peptide pool comprising the fractions after the end of the labeled protein peak and before the free dye peak;
- (c) depleting the protein pool by exposing the protein pool to an antibody depletion column and collecting column flow through as a depleted sample;
- (d) labeling the depleted sample with an uncharged thiol reactive label forming a saturated fluorescence labeled sample;
- (e) conducting two dimensional gel electrophoresis on the saturated fluorescence labeled sample, producing a 2D gel;
- (f) imaging the 2D gel and identifying a protein(s) of interest;
- and
- (g) conducting mass spectrometry analysis of the protein(s) of interest.
Dependencies are inferred from the claim text.