Method of diagnosing bacterial infections using bacterial glycoproteins
OwnerMario FELDMAN, Jeremy IWASHKIW +3inventors
InventorsMario FELDMAN, Jeremy IWASHKIW, Juan Esteban UGALDE, Andres Eduardo CIOCCHINI, Diego Jose COMERCI
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Abstract
The present application provides a method of diagnosing bacterial infections using engineered glycoproteins in an immunoassay. The engineered bacterial glycoproteins used in the immunoassay comprise a bacterial antigen covalently attached to a protein via polysaccharyltransferase (PTase)-mediated glycosylation, wherein the bacterial antigen is selected based on the bacterial infection of interest. Antibodies in a bodily fluid of subjects infected with a bacteria will bind to the engineered glycoprotein, and the resulting binding complexes are detected or quantitated.
Claims27
A method for diagnosing a bacterial infection in a subject comprising:
- (i) contacting a sample obtained from the subject with a mixture of one or more engineered glycoproteins;
- and
- (ii) detecting first binding complexes formed by binding of the glycoproteins by antibodies present in the sample that are specific for bacterial polysaccharides,
- wherein each of said glycoproteins comprises a bacterial antigen covalently attached to a protein via polysaccharyltransferase (PTase)-mediated glycosylation and wherein the presence of binding complexes is indicative of bacterial infection in the subject.
(canceled)
An engineered glycoprotein comprising an AcrA protein of Campylobacter jejuni glycosylated with an O-antigen of Yersinia pseudotuberculosis O9 (AcrA-AO9), an 0-antigen of E. coli O157 (AcrA-AO157) or an O-antigen of B. abortus (AcrA-OAg).
-16. (canceled)
An immunoassay kit for diagnosing a bacterial infection in a subject, the kit comprising:
- (a) a mixture of one or more engineered glycoproteins, wherein each glycoprotein comprises a bacterial antigen covalently attached to a protein via polysaccharyltransferase (PTase)-mediated glycosylation;
- and
- (b) instructions for contacting the mixture of engineered glycoproteins with a sample of bodily fluid from the subject and monitoring for formation of binding complexes formed by binding of the glycoproteins by antibodies in the sample that are specific for bacterial polysaccharides.
-24. (canceled)
-43. (canceled)
Dependencies are inferred from the claim text.