Electrophoretic Separation Devices and Methods for Using the Same
AssigneeThe Regents of the University of California
InventorsAmy E. Herr, Alex James Hughes, Elly Sinkala, Todd A. Duncombe, Kevin A. Yamauchi, Julea Vlassakis, Chi-Chih Kang, Zhuchen Xu, Robert Lin
Life of this patent
Estimating the term…
Abstract
Electrophoretic separation devices and methods for using the same are provided. Aspects of the devices include a polymeric separation medium that includes a plurality of microwells. Also provided are methods, systems and kits in which the subject devices find use. The devices and methods find use in a variety of different electrophoretic separation applications.
Claims20
A device comprising:
- a polymeric separation medium comprising a plurality of microwells, wherein the polymeric separation medium comprises functional groups that covalently bond to one or more sample components of interest in the separation medium upon application of an applied stimulus.
The device of any of the preceding claims, wherein the polymeric separation medium comprises 100 or more microwells.
The device of any of the preceding claims, wherein the microwells are dimensioned to accommodate single cells.
A method comprising:
- contacting a sample with a polymeric separation medium of any of the preceding claims;
- and
- applying an electric field to the polymeric separation medium in a manner sufficient to move at least some components of the sample from the microwell into the polymeric separation medium to produce separated sample components in the polymeric separation medium.
Dependencies are inferred from the claim text.