Method of identifying or evaluating synergistic combinations of actives and compositions containing the same
AssigneeThe Procter & Gamble Company
InventorsJohn Erich OBLONG, Holly Ann ROVITO
Life of this patent
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Abstract
A method of identifying or evaluating synergistic combinations of actives such as skin-care actives that improve the metabolism of a cell and provides an advantage over using the actives alone. The method includes contacting cells with test agents, alone and in combination, and determining a response of the cells to the test agent(s), wherein the response corresponds to a change in a metabolic indicator associated with glycolysis and a change in a metabolic indicator associated with oxidative phosphylation.
Claims20
A method of identifying or evaluating a synergistic combination of actives for use in a personal care composition, the method comprising:
- a. providing first, second and third test vessels, each test vessel comprising cells disposed in a medium;
- b. contacting the cells in the first test vessel with a first test agent;
- c. non-lethally detecting a metabolic indicator associated with each of glycolysis and oxidative phosphorylation to provide a response of each metabolic pathway to the first test agent;
- d. contacting the cells in the second test vessel with a second test agent;
- e. non-lethally detecting a metabolic indicator associated with each of glycolysis and oxidative phosphorylation to provide a response of each metabolic pathway to the second test agent;
- f. contacting the cells in the third test vessel with the first and second test agents in combination;
- g. non-lethally detecting a metabolic indicator associated with each of glycolysis and oxidative phosphorylation to provide a response of each metabolic pathway to the first and second test agents in combination;
- and
- h. identifying the combination of the first and second compositions as a synergistic combination of actives when at least one of the responses in (g) indicates a synergistic improvement over the corresponding response in (c) or (e).
A method of improving skin health, comprising:
- a. identifying a target area of skin in need of a skin-care benefit;
- and
- b. applying a cosmetically safe and effective amount of a personal care composition made according to the method of claim 14 to the target area.
A method of identifying or evaluating a synergistic combination of actives, comprising:
- a. providing a first test vessel comprising a first type of cells disposed in a first medium and a second test vessel comprising a second type of cells disposed in a second medium;
- b. providing a basal value for each of glycolysis and oxidative phosphorylation for the first type of cells and the second type of cells;
- c. contacting the first and second types of cells with a first test agent and a second test agent in combination;
- d. non-lethally detecting a metabolic indicator associated with each of glycolysis and oxidative phosphorylation in the first and second types of cells to provide a response of each metabolic pathway to the first and second test agents in combination;
- and
- e. identifying the combination of the first and second test agents as a synergistic combination of actives when at least one of the responses indicates a synergistic improvement over the corresponding basal value.
A personal care composition comprising a synergistic combination of skin-care actives, the composition comprising:
- a. a dermatologically acceptable carrier;
- b. a safe and effective amount of niacinamide as a first active;
- and
- c. a safe and effective amount of tocoquinone as a second active, wherein the safe and effective amount of niacinamide and tocoquinone in combination improves at least one of glycolysis and oxidative phosphorylation metabolism of a skin cell suffering from oxidative stress as a result of exposure to a stressor and provides a synergistic advantage over using the actives alone.
Dependencies are inferred from the claim text.