Mixer bypass sample injection for liquid chromatography
AssigneeAgilent Technologies, Inc.
InventorRobert William ALBRECHT, JR.
Life of this patent
Estimating the term…
Abstract
In a liquid chromatography system, a sample is injected into a column by flowing a solvent mixture from a mixer into the column along a solvent mixture flow path; and injecting a sample into the solvent mixture flow path downstream of the mixer. In another liquid chromatography system, a sample is injected into a column by loading an isolator fluid into a sample loop, loading a sample into the sample loop, and flowing the sample into the column as a plug in front of the isolator fluid.
Claims27
A liquid chromatography (LC) system, comprising:
- a column;
- a first solvent source;
- a second solvent source;
- a mixer communicating with the first solvent source via a first solvent line, with the second solvent source via a second solvent line, and with the column via a solvent mixture line;
- a fluidic junction in solvent mixture line;
- and
- an injection valve comprising a sample loop and communicating with the fluidic junction and an injection solvent source, wherein the injection valve is adjustable to a sample injection position that defines a flow path for an injection solvent through the sample loop and to the fluidic junction, such that the injection solvent carries a sample in the sample loop into the solvent mixture line downstream from the mixer.
(canceled)
(canceled)
(canceled)
A liquid chromatography (LC) system, comprising:
- a column;
- a sample source;
- an isolator fluid source;
- an injection valve comprising a sample loop and communicating with the sample source, the isolator fluid source, and a sample injection line leading to the column, wherein the injection valve is adjustable to an isolator fluid load position that defines a flow path for an isolator fluid to be flowed from the isolator fluid source into the sample loop;
- and
- a fluid moving device communicating with the sample loop and configured for selectively pulling fluid into the sample loop and pushing fluid out from the sample loop.
(canceled)
A method for injecting a sample into a liquid chromatography column, the method comprising:
- flowing a first solvent and a second solvent into a mixer to form a solvent mixture;
- flowing the solvent mixture into the column along a solvent mixture flow path;
- and
- while flowing the solvent mixture, injecting a sample comprising a sample material carried in an injection solvent into the solvent mixture flow path at a point between the mixer and the column.
-21. (canceled)
(canceled)
-34. (canceled)
Dependencies are inferred from the claim text.