Metabolic profiling in tissue and serum is indicative of tumor differentiation in prostate cancer
AssigneesDana-Farber Cancer Institute, Inc., The Brigham and Women's Hospital, Inc.
InventorsMassimo Loda, Kathryn L. Penney, Svitlana Tyekucheva
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Abstract
The invention provides methods and products to detect the presence of unidentified high grade prostate tumors in a subject with a Gleason score 7 prostate tumor. The method comprises obtaining a biological sample from a subject in need thereof, measuring a profile of metabolites in the biological sample, wherein the metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors, and classifying the profile of the metabolites to assign a grade to the sample based on the profile of the metabolites.
Claims30
A method to supplement Gleason score evaluation of a Gleason score 7 prostate tumor, the method comprising:
- obtaining a biological sample of a subject in need thereof;
- measuring a profile of metabolites in the biological sample, wherein the metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors;
- and
- classifying the profile of the metabolites to assign a supplemental Gleason grade to the sample based on the profile of the metabolites.
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8-9. (canceled)
11-13. (canceled)
A method to detect the presence of high grade prostate tumors in a subject with a Gleason score 7 prostate tumor, the method comprising:
- obtaining a biological sample of a subject in need thereof;
- measuring a profile of metabolites in the biological sample, wherein the metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors;
- and
- analyzing the profile of the metabolites with at least one processor programmed to implement a specific prediction model to assign a Gleason grade to the sample based on the profile of the metabolites.
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19-22. (canceled)
24-26. (canceled)
A method to supplement Gleason score evaluation of a Gleason 7 prostate tumor, the method comprising:
- classifying, with at least one processor, a profile of a set of metabolites in a biological sample obtained from a subject with a Gleason score 7 prostate tumor to assign a Gleason grade to the sample based on the profile of metabolites, wherein:
- metabolites in the set of metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors.
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32-41. (canceled)
A method, comprising:
- training, with at least one processor, a classifier to provide a trained classifier that distinguishes between Gleason score 6 and 8, wherein the classifier is trained using a training set of samples comprising profiles of the metabolites that are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors.
45-47. (canceled)
A computer-readable storage medium encoded with a plurality of instructions that, when executed by at least one processor, performs a method comprising:
- classifying a profile of a set of metabolites in a biological sample obtained from a subject with a Gleason score 7 prostate tumor to assign a Gleason grade to the sample based on the profile of metabolites, wherein:
- metabolites in the set of metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors.
49-50. (canceled)
Dependencies are inferred from the claim text.