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US 2015/0309294 A1Published application
Single Plane Illumination Microscope
AssigneeEuropaeisches Laboratorium Fuer Molekularbiologie (EMBL)
InventorErnst H.K. STELZER
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Abstract
The invention relates to a microscope in which a layer of the sample is illuminated by a plurality of thin strips of light (11) passed through a grid (34) and the sample is viewed (5) perpendicular to the plane of the strips of light. To record the image, the object (4) is displaced through the strips of light (11). At least three different images of the objects (4) are made at different phase angles. The images can be combined to form a single combined image.
Claims27
A microscope having at least one illumination beam path and at least one detection beam path, wherein
- the at least one each illumination beam path is provided with a focusing arrangement for producing a two-dimensional object illumination pattern which extends in the direction of an illumination axis of the at least one illumination beam path and transversely thereto,
- a detection direction of the at least one detection beam path is substantially orthogonal to the two-dimensional object illumination region, and
- a mobile arrangement is provided for producing a relative movement between the two-dimensional object illumination pattern and an object to be studied.
Dependencies are inferred from the claim text.