Linear movement type reaction treatment apparatus and method thereof

A linear movement type reaction treatment method comprising:

• arranging at least one reaction container and two or more liquid storage portions as a container group in at least one linear shape;
• detachably attaching one or two or more dispensing tips to a dispensing head;
• moving the dispensing head in the linear arrangement direction with respect to the container group;
• storing a sample suspension in a sample storage portion, as the sample storage portion at least one of the liquid storage portions are selected by using the dispensing tip;
• applying an ultrasonic vibration to the sample storage portion;
• and
• transferring each sample suspension in the linear arrangement direction to the next liquid storage portion disposed in a linear shape or the reaction container by using the dispensing tip,
• wherein the step of arranging comprises a step of providing two or more exclusive regions corresponding to two or more sets of dispensing tips respectively so that one set of dispensing tips enter and the other sets of dispensing tips do not enter in the container group;
• and arranging at least one reaction container, two or more liquid storage portions storing a magnetic particle suspension and a solution necessary for treatment, and one or two or more tip storage portions storing one or two or more dispensing tips in an attachable manner in the linear shape in each exclusive region,
• wherein the step of detachably attaching comprises a step of detachably attaching each set of dispensing tips to the dispensing head,
• wherein the step of moving the dispensing head comprises a step of relatively moving the dispensing head in the linear arrangement direction inside each exclusive region all together, the step of applying an ultrasonic vibration comprises a step of applying an ultrasonic vibration to a sample storage portion, as the sample storage portion at least one of the liquid storage portions are selected in each exclusive region, and
• wherein the method further comprises:
• transferring each scattering prevention lid stored in a position in the linear arrangement direction while each scattering prevention lid is attached to the dispensing head, after storing each sample suspension in each sample storage portion, attaching the scattering prevention lid to the opening portion of the sample storage portion so as to close the opening portion, by using the dispensing head, applying an ultrasonic vibration to each sample storage portion, after detaching the scattering prevention lid from the dispensing head, and punching a film provided with the scattering prevention lid by downward movement of the punching tip detachably attached to the dispensing head and a sample suspension can be taken out by the dispensing tip attached to the dispensing head.

A linear movement type reaction treatment method comprising:

• detachably attaching a dispensing tip to a dispensing head;
• relatively moving the dispensing head and a container group in a linear arrangement direction so as to separate a target material using a magnetic particle suspension having magnetic particles suspended to capture at least a target material, a sample suspension stored in a sample storage portion to which an ultrasonic vibration is applied, and a separation/extraction solution of a target material arranged in a linear shape in the container group;
• introducing the separated target material and a reaction solution used for a reaction into a plurality of reaction contains located in the linear arrangement direction of the container group;
• moving a light guiding trestle provided in the dispensing head and including two or more link portions provided with front ends of one or two or more light guiding portions with respect to a reaction container along with the dispensing head;
• directly or indirectly linking the reaction containers to the link portion all together so as to optically connect the inside of the linked reaction container to the light guiding portion;
• performing temperature control inside the reaction container;
• and
• guiding light from the reaction container to a connection end array body supporting two or more connection ends provided with the rear ends of the light guiding portions having the front ends provided in the link portion and provided so as to correspond to each link portion in a predetermined path, and sequentially and optically connecting the connection ends to one or two or more measurement ends provided in a measurement unit provided so as to be adjacent to or contact the arrangement surface by the relative movement thereof, so that light based on an optical state inside the reaction container in accordance with the sequential optical connection in the predetermined path is received by the measurement unit,
• wherein a step of applying the ultrasonic vibration to the sample storage portion comprises:
• transferring each scattering prevention lid stored in a position in the linear arrangement direction while each scattering prevention lid is attached to the dispensing head, after storing each sample suspension in each sample storage portion;
• attaching the scattering prevention lid to the opening portion of the sample storage portion so as to close the opening portion by using the dispensing head;
• applying an ultrasonic vibration to each sample storage portion, after detaching the scattering prevention lid from the dispensing head;
• and
• punching a film provided with the scattering prevention lid by downward movement of the punching tip detachably attached to the dispensing head and a sample suspension can be taken out by the dispensing tip attached to the dispensing head.