Circulating proteolytic biomarkers of cell death and methods for the use thereof
AssigneeThe Regents of the University of California
InventorsJames A. Wells, Arun P. Wiita
Life of this patent
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Abstract
Provided herein are peptides useful, inter alia, for determining a level of apoptosis in a cancer patient. Further provided are complexes including said peptides bound to a binding reagent and antibodies specifically binding said peptides.
Claims17
A method of determining a level of a peptide in a cancer subject, the method comprising:
- (i) assaying a biological sample from a cancer subject;
- and
- (ii) determining a level of a peptide of SEQ ID NO:442, SEQ ID NO:444, SEQ ID NO:491, SEQ ID NO:506, SEQ ID NO:517, SEQ ID NO:520, SEQ ID NO:522, SEQ ID NO:527, SEQ ID NO:533, SEQ ID NO:541, SEQ ID NO:548 or SEQ ID NO:552 in said biological sample.
A method of determining apoptosis in a subject, the method comprising:
- (i) detecting a level of a peptide of SEQ ID NO:442, SEQ ID NO:444, SEQ ID NO:491, SEQ ID NO:506, SEQ ID NO:517, SEQ ID NO:520, SEQ ID NO:522, SEQ ID NO:527, SEQ ID NO:533, SEQ ID NO:541, SEQ ID NO:548 or SEQ ID NO:552 in a biological sample from a subject receiving or having received a therapeutic agent, wherein said detecting comprises contacting a binding reagent with the peptide to form a binding reagent peptide complex and detecting the binding reagent peptide complex;
- and
- (ii) comparing said level to a standard control, thereby determining apoptosis in a subject.
An in vitro polypeptide complex comprising a peptide bound to a binding reagent, wherein said peptide is SEQ ID NO:442, SEQ ID NO:444, SEQ ID NO:491, SEQ ID NO:506, SEQ ID NO:517, SEQ ID NO:520, SEQ ID NO:522, SEQ ID NO:527, SEQ ID NO:533, SEQ ID NO:541, SEQ ID NO:548 or SEQ ID NO:552.
A conjugate comprising a peptide covalently bound to a detectable moiety, wherein said peptide is SEQ ID NO:442, SEQ ID NO:444, SEQ ID NO:491, SEQ ID NO:506, SEQ ID NO:517, SEQ ID NO:520, SEQ ID NO:522, SEQ ID NO:527, SEQ ID NO:533, SEQ ID NO:541, SEQ ID NO:548 or SEQ ID NO:552.
Dependencies are inferred from the claim text.