Fluorogenic dendrimer reporters and related methods of use
AssigneeThe Regents of the University of Michigan
InventorsJames R. Baker, JR., Hong Zong, Sascha Goonewardena
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Abstract
The present invention relates to fluorogenic dendrimer reporters. In particular, the present invention relates to dendrimer nanoparticles conjugated with ‘click-on’ fluorogenic reporters and related methods of use.
Claims19
1-37. (canceled)
A composition comprising a dendrimer nanoparticle conjugated with a fluorogenic reporter, wherein said fluorogenic reporter is conjugated with an alkyne chemical moiety, wherein said dendrimer nanoparticle is optionally conjugated with one or more additional functional agents independently selected from the group consisting of a therapeutic agent, a targeting agent, an imaging agent, and a trigger agent.
A method of tracking a small molecule within a biological sample, comprising
- providing a dendrimer nanoparticle and a functionalized small molecule, wherein either i) said dendrimer nanoparticle is conjugated with an azido based ‘click-on’ fluorogenic reporter and said functionalized small molecule is an alkyne functionalized small molecule, or ii) said dendrimer nanoparticle is conjugated with an alkyne based ‘click-on’ fluorogenic reporter and said functionalized small molecule is an azido functionalized small molecule, and
- introducing said dendrimer nanoparticle and functionalized small molecule into a biological sample,
- detecting fluorescence from said dendrimer nanoparticle upon interaction between said dendrimer nanoparticle with said functionalized small molecule, wherein said interaction results in formation of a triazole ring via a 1,3-dipolar cycloaddition reaction, wherein said formation of said triazole ring results in detectable fluorescence from said ‘click-on’ fluorogenic reporter, and
- tracking said functionalized small molecule via fluorescence from said ‘click-on’ fluorescence reporter.
A method of monitoring a biological process within a biological sample, comprising
- providing a dendrimer nanoparticle and a functionalized small molecule, wherein either i) said dendrimer nanoparticle is conjugated with an azido based ‘click-on’ fluorogenic reporter and said functionalized small molecule is an alkyne functionalized small molecule, or ii) said dendrimer nanoparticle is conjugated with an alkyne based ‘click-on’ fluorogenic reporter and said functionalized small molecule is an azido functionalized small molecule, and
- introducing said dendrimer nanoparticle and functionalized small molecule into a biological sample,
- detecting fluorescence from said dendrimer nanoparticle upon interaction between said dendrimer nanoparticle with said functionalized small molecule, wherein said interaction results in formation of a triazole ring via a 1,3-dipolar cycloaddition reaction, wherein said formation of said triazole ring results in detectable fluorescence from said ‘click-on’ fluorogenic reporter,
- tracking said functionalized small molecule via fluorescence from said ‘click-on’ fluorescence reporter, and
- monitoring a biological process associated with said functionalized small molecule through tracking said detectable fluorescence.
Dependencies are inferred from the claim text.