Biomarker panel for prediction of recurrent colon cancer
AssigneeNuclea Biotechnologies, Inc.
InventorPatrick J. Muraca
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Abstract
The present invention provides a biomarker panel predictive of whether colorectal cancer is likely to recur or metastasize in an afflicted patient. By identifying the likelihood of recurrence, a treatment provider may determine in advance those patients who would benefit from certain types of treatment. The present invention further provide methods of identifying gene and protein expression profiles associated with the likelihood of recurrence/metastasis of colorectal cancer in a patient sample.
Claims9
1-20. (canceled)
A method of determining if a colon cancer patient's colon cancer is likely to recur, comprising
- a. obtaining a tumor sample from the colon cancer patient;
- and
- b. determining the expression levels in the sample of the following proteins:
- phospho-AIK comprising the amino acid sequence of SEQ ID NO. 8, phospho-mTOR comprising the amino acid sequence of SEQ ID NO. 9, phospho MAPK comprising the amino acid sequence of SEQ ID NO. 10, phospho-MEK comprising the amino acid sequence of SEQ ID NO. 11, phospho-S6 comprising the amino acid sequence of SEQ ID NO. 12, AKT comprising the amino acid sequence of SEQ ID NO. 13, and SSTR1 comprising the amino acid sequence of SEQ ID NO. 14;
- wherein expression of the proteins is up-regulated in patients whose colon cancer is not likely to recur compared to expression of these proteins in patients whose cancer is likely to recur.
An assay for determining if a colon cancer patient's colon cancer is likely to recur, comprising monoclonal specific for the following proteins:
- phospho-mTOR comprising the amino acid sequence of SEQ ID NO. 9, phospho-AIK comprising the amino acid sequence of SEQ ID NO. 8, phospho-MEK comprising the amino acid sequence of SEQ ID NO. 11, phospho-S6 comprising the amino acid sequence of SEQ ID NO. 12, AKT comprising the amino acid sequence of SEQ ID NO. 13, SSTR1 comprising the amino acid sequence of SEQ ID NO. 14, and phospho-MAPK comprising the amino acid sequence of SEQ ID NO. 10;
- wherein the monoclonal antibodies are specifically reactive with phosphorylated forms of said proteins.
An assay kit comprising monoclonal antibodies specific for an epitope of the following proteins:
- phospho-AIK comprising the amino acid sequence of SEQ ID NO. 8, phospho-mTOR comprising the amino acid sequence of SEQ ID NO. 9, phospho MAPK comprising the amino acid sequence of SEQ ID NO. 10, phospho-MEK comprising the amino acid sequence of SEQ ID NO. 11, phospho-S6 comprising the amino acid sequence of SEQ ID NO. 12, AKT comprising the amino acid sequence of SEQ ID NO. 13, and SSTR1 comprising the amino acid sequence of SEQ ID NO. 14.
Dependencies are inferred from the claim text.