Synthetic Ligands for the Differentiation of Closely Related Toxins and Pathogens
AssigneeUniversity of Cincinnati
InventorsSuri Saranathan Iyer, Duane Michael Hatch, Ramesh Ratan Kale, Allison Ann Weiss, Shantini D. Gammage, Colleen McGannon O'Malley
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Abstract
Synthetic ligand compounds and methods of differentiating between Shiga toxin 1 and Shiga toxin 2 are disclosed herein. Another embodiment includes a kit for differentiating between Shiga toxin 1 and Shiga toxin 2. Assay systems and methods for providing an assay are also provided for herein.
Claims13
A compound for detecting variant toxins wherein said toxin is selected from the group consisting of Shiga toxin 1 and Shiga toxin 2, the compound comprising the general formula (I):
- wherein:
- n equals 1, 2, 3, 4, 5 or 6,
- B=O, NH, S, SO, SO2, or P(O)R,
- C=NH2, COOH, biotin or derivatives thereof, and
- A comprises a glycoconjugate, wherein the glycoconjugate is selected from the group consisting of:
- wherein R comprises H, Ac or derivatives thereof;
- X=OH, SH, NHAc, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof;
- Y=OH, NHAc, SH, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof;
- Z=OH, NHAc, SH, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof.
4-7. (canceled)
9-11. (canceled)
A glycoconjugate consisting essentially of:
- wherein R comprises H, Ac or derivatives thereof;
- X=OH, SH, NHAc, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof;
- Y=OH, NHAc, SH, NHCF3, NH2 NHCH(═NH)NH2 or derivatives thereof;
- Z=OH, NHAc, SH, NHCF3, NH2, NHCH(═NH)NH2 or derivatives thereof, and wherein the glycoconjugate has a sufficient affinity to bind to a Shiga toxin for enterotoxigenic E. coli, wherein (b) binds with Shiga toxin 1 and (a) and (c) bind with Shiga toxin 2.
A kit for detecting a toxin, the kit comprises at least one container containing at least one capture agent, wherein the at least one capture agent substantially only binds to Shiga toxin 1 or Shiga toxin 2.
16-21. (canceled)
Dependencies are inferred from the claim text.