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US 2015/0309011 A1Published application
Microfluidic device
AssigneeCalifornia Institute of Technology
InventorsYu-Chong Tai, Siyang Zheng, Jeffrey Chun-Hui Lin, Harvey L. Kasdan
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Abstract
Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.
Claims26
1-27. (canceled)
A method for analyzing cells, the method comprising:
- a) introducing a blood sample comprising fluorescently labeled cells and fluorescently labeled control beads to an inlet of a first microfluidic channel that gradually narrows, wherein the fluorescently labeled cells are labeled with a fluorophore different than a fluorophore label of the control beads;
- b) flowing the blood sample from the inlet of the first microfluidic channel to an outlet of a second microfluidic channel, wherein the second microfluidic channel is directly coupled to the first microfluidic channel;
- c) exciting the fluorophores of the fluorescently labeled cells and of the fluorescently labeled control beads using an excitation source;
- d) detecting fluorescence of the fluorescently labeled cells and fluorescence of the fluorescently labeled control beads as they pass through a detection zone in the second microfluidic channel, wherein the detection is performed on individual cells;
- e) measuring the fluorescence of the fluorescently labeled cells and of the fluorescently labeled beads using a light sensor;
- and
- f) using the measured fluorescence to thereby provide a result concerning the blood sample.
4 more elements
Dependencies are inferred from the claim text.