Methods of detecting reverse triiodothyronine by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsJ. Fred BANKS, Peter P. CHOU, Noriya M. MATT
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Abstract
Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.
Claims24
A method for determining the amount of reverse triiodothyronine (rT3) in a sample by tandem mass spectrometry, said method comprising:
- a. subjecting the rT3 from the sample to liquid chromatography;
- b. ionizing rT3 from the sample and an internal standard to generate at least one rT3 ion and at least one internal standard ion detectable by tandem mass spectrometry;
- c. determining the amount of said at least one rT3 ion and the amount of said at least one internal standard ion by tandem mass spectrometry;
- and
- d. determining the amount of rT3 in the sample, comprising comparing the amount of said at least one rT3 ion and the amount of said at least one internal standard ion.
The method of claim 4, wherein said protein precipitation comprises organic solvent precipitation.
A method for determining the amount of reverse triiodothyronine (rT3) in a sample by tandem mass spectrometry, said method comprising:
- a. subjecting the rT3 from the sample to liquid chromatography;
- b. ionizing rT3 from the sample to generate at least one rT3 ion detectable by tandem mass spectrometry;
- c. determining the amount of said at least one rT3 ion by tandem mass spectrometry;
- and
- d. relating the amount of rT3 in the sample from the amount of said at least one rT3 ion.
Dependencies are inferred from the claim text.