Method of spatial high resolution imaging of a structure of a sample, the structure comprising a luminophore

A method of spatial high resolution imaging of a structure of a sample, the structure comprising a luminophore having an electronic ground state and a luminescent state into which the luminophore, out of its electronic ground state, is excitable by luminescence excitation light, the method comprising

• in the measurement area, subjecting the sample to an intensity distribution of luminescence inhibiting light comprising a local minimum, wherein the luminescence inhibiting light is configured to disturb the electronic ground state of the luminophore to such an extent that the luminophore, in its disturbed electronic ground state, has an absorption cross-section for the luminescence excitation light which is reduced by at least 50% as compared to an absorption cross-section for the luminescence excitation light of the luminophore its undisturbed electronic ground state;
• in the measurement area, subjecting the sample to the luminescence excitation light exciting the luminophore out of its undisturbed electronic ground state into its luminescent state;
• registering luminescence light emitted out of the measurement area;
• assigning the registered luminescence light to a position of the local minimum within the sample;
• scanning the sample with the local minimum of the intensity distribution of the luminescence inhibiting light;
• and
• repeating the steps of subjecting, registering and assigning for a plurality of positions of the local minimum of the intensity distribution of the luminescence inhibiting light within the sample.