Compositions and methods for detecting human papillomavirus nucleic acid
AssigneeGen-Prob Incorporated
InventorAstrid R.W. SCHRODER
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Abstract
Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of a human papillomavirus (HPV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
Claims91
An oligomer combination for detecting a human papillomavirus type 33 (HPV33) target nucleic acid in a sample suspected of containing HPV33 and at least one of HPV types 31, 52, and 58, said oligomer combination comprising:
- first and second amplification oligomers for specifically amplifying an HPV33 nucleic acid target region, wherein (a) the first HPV33 amplification oligomer comprises a first target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides contained in the sequence of SEQ ID NO:
- 66 and that includes at least the sequence of SEQ ID NO:
- 67, SEQ ID NO:
- 68, or SEQ ID NO:
- 69;
- and (b) the second HPV33 amplification oligomer comprises a second target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides contained in the sequence of SEQ ID NO:
- 70 and that includes at least the sequence of SEQ ID NO:
- 71, SEQ ID NO:
- 72, or SEQ ID NO:
- 73.
An oligomer combination for detecting a human papillomavirus type 33 (HPV33) target nucleic acid in a sample, said oligomer combination comprising:
- first and second amplification oligomers for amplifying an HPV33 nucleic acid target region, wherein (a) the first HPV33 amplification oligomer comprises a first target-hybridizing sequence selected from the group consisting of SEQ ID NOs:
- 1-8;
- and (b) the second HPV33 amplification oligomer comprises a second target-hybridizing sequence selected from the group consisting of SEQ ID NOs:
- 9-16.
The oligomer combination of claim 21, wherein the HPV33 detection probe target-hybridizing sequence is selected from the group consisting of SEQ ID NOs:
- 54-58.
A method for detecting a human papillomavirus type 33 (HPV33) target nucleic acid in a sample suspected of containing HPV33 and at least one of HPV types 31, 52, and 58, said method comprising:
- (a) providing a sample, wherein said sample is suspected of containing HPV33 and at least one of HPV types 31, 52, and 58;
- (b) contacting the sample with an oligomer combination for specifically amplifying an HPV33 nucleic acid target region, said oligomer combination comprising (i) a first HPV33 amplification oligomer comprising a first target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides contained in the sequence of SEQ ID NO:
- 66 and that includes at least the sequence of SEQ ID NO:
- 67, SEQ ID NO:
- 68, or SEQ ID NO:
- 69;
- and (ii) a second HPV33 amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 27 contiguous nucleotides contained in the sequence of SEQ ID NO:
- 70 and that includes at least the sequence of SEQ ID NO:
- 71, SEQ ID NO:
- 72, or SEQ ID NO:
- 73;
- (c) performing an in vitro nucleic acid amplification reaction, wherein any HPV33 target nucleic acid present in said sample is used as a template for generating an HPV33 amplification product;
- and
- (d) detecting the presence or absence of the HPV33 amplification product, thereby indicating the presence or absence of HPV33 in said sample.
A method for detecting a human papillomavirus type 33 (HPV33) target nucleic acid in a sample, said method comprising:
- (a) providing a sample, wherein said sample is suspected of containing HPV33;
- (b) contacting the sample with an oligomer combination for amplifying an HPV33 nucleic acid target region, said oligomer combination comprising (i) a first HPV33 amplification oligomer comprising a first target-hybridizing sequence selected from the group consisting of SEQ ID NOs:
- 1-8;
- and (b) a second HPV33 amplification oligomer comprising a second target-hybridizing sequence selected from the group consisting of SEQ ID NOs:
- 9-16;
- (c) performing an in vitro nucleic acid amplification reaction, wherein any HPV33 target nucleic acid present in said sample is used as a template for generating an HPV33 amplification product;
- and
- (d) detecting the presence or absence of the HPV33 amplification product, thereby indicating the presence or absence of HPV33 in said sample.
Dependencies are inferred from the claim text.