Epigenetic method for the identification of subpopulations of cd8+ t lymphocytes, in particular cd8 alpha and beta t lymphocytes
AssigneeEpiontis GmbH
InventorSven OLEK
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Abstract
The present invention relates to a method, in particular an in vitro method, for identifying CD8 positive subpopulations of a mammal, wherein said method comprises analyzing the bisulfite convertibility of at least one CpG position in the CD8 beta and CD8 alpha cell specific bisulfite convertibility gene region according to SEQ ID No. 1 and 2, wherein a bisulfite convertibility of at least one CpG position in said gene regions is indicative for a CD3+CD8+ and/or CD3+/−CD8+ cell. The analyses according to the invention can identify CD3+CD8+ and/or CD3+/−CD8+ cells on an epigenetic level and distinguish them from all other cells in complex samples, such as, for example, other blood cells.
Claims23
A method for identifying a subpopulation of cytotoxic T cells in a mammal, comprising analyzing the bisulfite convertibility of at least one CpG position in a gene selected from the group of CD8+ alpha and CD8+ beta, wherein a bisulfite convertibility of at least one CpG position in the CD8+ beta gene is indicative for a CD3+CD8+ cytotoxic T cell, and a bisulfite convertibility of the CD8+ alpha gene is indicative for a CD3+/−CD8+ cytotoxic T cell.
An oligomer according to any of SEQ ID NOs:8-13, or an amplicon according to any of SEQ ID NOs:3-7.
23-24. (canceled)
Dependencies are inferred from the claim text.