Non-mass determined base compositions for nucleic acid detection
AssigneeIbis Biosciences, Inc.
InventorChristopher C. Sappenfield
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Abstract
The present invention provides systems, methods, and compositions for nucleic acid detection based on non-mass determined base compositions. For example, in certain embodiments, base count data for a template nucleic acid is generated using an approach that does not measure molecular mass of the template nucleic acid (e.g., by sequencing the template nucleic acid) and a database comprising base count entries is queried to identify the target nucleic acid. In particular embodiments, sequencing is employed which is conducted in substantially real-time.
Claims1
A method of identifying a template nucleic acid, the method comprising:
- (a) determining at least a partial base count of at least a subsequence of at least one template nucleic acid and/or a complement thereof, using an approach that does not measure molecular mass of the template nucleic acid, to produce base count data;
- and
- (b) querying a database comprising at least one base count entry corresponding to an identified nucleic acid to produce a match of the base count data with the base count entry, thereby identifying the template nucleic acid.
Dependencies are inferred from the claim text.