Methods for Analyzing Minute Cellular Nucleic Acids
AssigneeFluidigm Corporation
InventorsFatih Ozsolak, Patrice M. Milos
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Abstract
The invention generally relates to methods for analyzing cellular nucleic acid. Methods of the invention involve capturing RNA from a lysed cell onto a substrate, producing a cDNA/RNA duplex, removing the RNA from the cDNA/RNA duplex, priming the cDNA to produce a primer/cDNA duplex, exposing the primer/cDNA duplex to at least one detectably labeled nucleotide in the presence of a polymerase capable of catalyzing addition of the nucleotide to the primer/cDNA duplex, detecting incorporation of the nucleotide into the primer portion, and repeating the exposing and detecting steps at least once.
Claims21
A method for analyzing cellular nucleic acid, the method comprising the steps of:
- capturing RNA from a lysed cell onto a substrate;
- producing a cDNA/RNA duplex;
- removing the RNA from the cDNA/RNA duplex;
- priming the cDNA to produce a primer/cDNA duplex;
- exposing the primer/cDNA duplex to at least one detectably labeled nucleotide in the presence of a polymerase capable of catalyzing addition of the nucleotide to the primer/cDNA duplex;
- detecting incorporation of the nucleotide into the primer portion;
- and
- repeating the exposing and detecting steps at least once.
Dependencies are inferred from the claim text.