Apparatus for centrifuge mountable manifold for processing fluidic assays

A detection system, comprising:

• a motor;
• a flowrotor;
• and
• a controller in communication with the motor, the controller configured to provide the motor with instructions comprising:
• rotate the flowrotor in a first direction with a first radial acceleration to move an assay from the inlet to a first reaction chamber of the flowrotor;
• retain the assay at the first reaction chamber by maintaining a first angular velocity to induce a reaction with a first reactant to thereby provide a reacted assay;
• accelerate the flowrotor's rotation at a negative angular acceleration relative to the first angular velocity until the first angular velocity is substantially reversed thereby moving the reacted assay from the first reaction chamber to an evaluation chamber.

A computer-readable storage device containing a set of instructions to cause a motor to perform a process comprising:

• rotate a flowrotor in a first direction with a first radial acceleration to move an assay from the inlet to a first reaction chamber, the first reaction chamber having an inlet and an outlet and containing a first reactant;
• retain the assay at the first reaction chamber for a first duration by maintaining a first angular velocity to induce a reaction with the first reactant and provide a reacted assay;
• accelerate the flowrotor at a negative angular acceleration relative to the first angular velocity until the angular velocity is reversed thereby moving the reacted assay from the first reaction chamber to a second chamber;
• retain the reacted assay at the second chamber for a second duration to induce a reaction with a second reagent to form a final assay;
• and
• rotate the flowrotor at a second radial acceleration to move the final assay to an evaluation chamber.