Methods of Detecting Target Nucleic Acids
AssigneeApplied Biosystems, LLC
InventorsMuhammad A. SHARAF, Timothy WOUDENBERG, Khairuzzaman Bashar MULLAH
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Abstract
The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.
Claims21
1-73. (canceled)
A method of detecting a target polynucleotide, comprising:
- a) contacting a coded molecule with a target polynucleotide, wherein the coded molecule comprises
- (i) one or more block polymer regions, and
- (ii) a target probe capable of hybridizing to the target polynucleotide;
- b) modifying the coded molecule by modifying the target probe with a modifying agent following hybridizing of the target probe with the target polynucleotide, wherein the modification is dependent on the target polynucleotide hybridized to the target probe;
- c) translocating the modified coded molecule through a nanopore and detecting a signal pattern associated with the modified coded molecule;
- and
- d) comparing the detected signal pattern to a signal pattern of an unmodified coded molecule, wherein a difference in the detected signal pattern of the modified coded molecule compared to the signal pattern of the unmodified coded molecule indicates the presence of the target polynucleotide.
Dependencies are inferred from the claim text.