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US 2015/0307923 A1Published application
Megasphaera cerevisiae system process control (spc) primers, probes, and methods
AssigneeFred Hutchinson Cancer Research Center
InventorsDavid FREDRICKS, Daisy KO
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Abstract
Provided are system process control (SPC) compositions and methods for confirming DNA extraction and PCR amplification of a patient sample through the detection of an introduced control organism, such as Megasphaera cerevisiae, which is not otherwise present in a patient sample. Compositions of the present disclosure include primers, primer sets, and probes for specifically detecting a control organism introduced into a patient sample.
Claims21
A system process control (SPC) method, comprising:
- (a) introducing a control bacterium into a patient sample;
- (b) carrying out a PCR reaction on the patient sample to generate a PCR amplicon that comprises a region of the control bacterium's genome, wherein the PCR reaction uses at least one primer pair including a forward primer and a reverse primer wherein each of the forward and reverse primers is complementary to a region of the control bacterium's genome;
- and
- (c) detecting the PCR amplicon.
1 more element
21-56. (canceled)
Dependencies are inferred from the claim text.