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US 2015/0307910 A1Published application
Methods and systems for fast pcr heating
AssigneeBjs IP Ltd
InventorsIan GUNTER, Richard LEWIS
Life of this patent
Estimating the term…
Abstract
A microplate for polymerase chain reaction (PCR) comprises a substrate having a metallic material for heating PCR samples, and a barrier layer disposed adjacent to the substrate. In some cases, the barrier layer is formed of a first polymeric material. The microplate includes one or more wells for containing PCR samples. The one or more wells are formed of a second polymeric material sealed to the barrier layer. In some cases, the substrate can provide a PCR ramp rate of at least about 5° C./second.
Claims22
A method for processing a biological sample, comprising:
- (a) providing a microplate comprising (i) a substrate having a metallic material, (ii) a barrier layer coated on said substrate, said barrier layer formed of a first polymeric material, (iii) a moulding comprising one or more wells sealed to said barrier layer, said one or more wells formed of a second polymeric material that is separate from said first polymeric material, and (iv) electrodes as projections from said substrate in electrical communication with said substrate;
- (b) depositing said biological sample in a solution in said one or more wells;
- and
- (c) directing electrical current from said electrodes through said substrate to subject said solution to one or more heating cycles, which one or more heating cycles comprise heating said solution at a heating rate of at least about 5° C./second upon flow of said electrical current through said substrate.
1 more element
A method for processing a biological sample, comprising:
- (a) providing a microplate comprising (i) a substrate having a metallic material, (ii) a barrier layer coated on said substrate, said barrier layer formed of a first polymeric material, (iii) a moulding comprising one or more wells sealed to said barrier layer, said one or more wells formed of a second polymeric material that is separate from said first polymeric material, and (iv) electrodes as projections from said substrate in electrical communication with said substrate;
- (b) depositing said biological sample in a solution in said one or more wells;
- and
- (c) directing electrical current from said electrodes through said substrate to subject said solution to one or more heating cycles at a rate of at least about 1 heating cycle per 30 seconds.
1 more element
Dependencies are inferred from the claim text.