Back to results
US 2015/0307908 A1Published application
Compositions Comprising A Polypeptide Having Cellulolytic Enhancing Activity And A Bicyclic Compound And Uses Thereof
AssigneeNovozymes, Inc.
InventorsJason Quinlan, Feng Xu, Matthew Sweeney
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a bicyclic compound. The present invention also relates to methods of using the compositions.
Claims24
A method for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition in the presence of a GH61 polypeptide having cellulolytic enhancing activity and a bicyclic compound;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation,
- wherein the bicyclic compound is of formula (I) or (II):
- wherein the bond indicated with a dashed line can be single or double;
- R1 and R2 are independently hydrogen, —C(O)R5, —C(O)OR6, —C(O)NHR7, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- R3, R4a, R4b, and R4c are independently hydrogen, halogen, —OH, —OR8, —CN, —NO2, —N(R9)(R10), —C(O)R5, —C(O)OR6, —C(O)NHR7, —OC(O)R11, —NHC(O)R12, —OC(O)OR13, —NHC(O)OR14, —OC(O)NHR15, —NHC(O)NHR16, —SO2R17, —SO2N(R18)(R19), —SR20, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and wherein two of R4a, R4b, and R4c may together form an optionally substituted fused ring;
- R5, R6, R7, R8, R9, R10, R11, R12, R13, R14, R15, R16, R18, R19, and R20 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and
- R17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- or a salt or solvate thereof;
- wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the bicyclic compound enhances hydrolysis of a cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:
- GH
-
-
- 61
-
-
- effect
- =
- %
-
-
- conversion
- (
- +
- GH
-
-
- 61
- )
- %
-
-
- conversion
- (
- no
-
-
- GH
-
-
- 61
- )
- .
44 more elements
The method of claim 1, wherein the bicyclic compound is of the formula (I-A) or (II-A):
- wherein R1, R2, R4a, and R8 are as defined in claim 8;
- or a salt or solvate thereof.
The method of claim 1, wherein the bicyclic compound is of the formula (I-B) or (II-B):
- wherein R1, R2, and R4a are as defined in claim 8;
- or a salt or solvate thereof.
The method of claim 1, wherein the bicyclic compound is of the formula (I-C), (I-D), (I-E), or (I-F):
- wherein R1, R2, R3, R4a, R4b, and R4c are as defined in claim 8;
- or a salt or solvate thereof.
A method of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of a polypeptide having cellulolytic enhancing activity and a bicyclic compound,
- wherein the bicyclic compound is of formula (I) or (II):
- wherein the bond indicated with a dashed line can be single or double;
- R1 and R2 are independently hydrogen, —C(O)R5, —C(O)OR6, —C(O)NHR7, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- R3, R4a, R4b, and R4c are independently hydrogen, halogen, —OH, —OR8, —CN, —NO2, —N(R9)(R10), —C(O)R5, —C(O)OR6, —C(O)NHR7, —OC(O)R11, —NHC(O)R12, —OC(O)OR13, NHC(O)OR14, —OC(O)NHR15, —NHC(O)NHR16, —SO2R17, —SO2N(R18)(R19), —SR20, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and wherein two of R4a, R4b, and R4c may together form an optionally substituted fused ring;
- R5, R6, R7, R8, R9, R10, R11, R12, R13, R14, R15, R16, R18, R19, and R20 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- and
- R17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
- or a salt or solvate thereof;
- wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the bicyclic compound enhances hydrolysis of a cellulosic material by the enzyme composition according to the following formula with a ratio greater than 1:
- GH
-
-
- 61
-
-
- effect
- =
- %
-
-
- conversion
- (
- +
-
- GH
-
-
- 61
- )
- %
-
-
- conversion
- (
- no
-
-
- GH
-
-
- 61
- )
- .
42 more elements
Dependencies are inferred from the claim text.