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US 2015/0307907 A1Published application
Method for Producing L-Amino Acid
AssigneeAjinomoto Co., Inc.
InventorsSeiko Hirano, Kazuyuki Hayashi
Life of this patent
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Abstract
A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a coryneform bacterium having an L-amino acid-producing ability, which is modified so that the activity of a phosphate transporter is increased, in a medium, and collecting the L-amino acid from the medium.
Claims20
A method for producing an L-amino acid comprising:
- A) culturing a coryneform bacterium having an L-amino acid-producing ability in a medium;
- and
- B) collecting the L-amino acid from the medium,
- wherein the bacterium has been modified so that the activity of a phosphate transporter is increased.
1 more element
A method for producing an L-amino acid comprising:
- A) culturing a coryneform bacterium having an L-amino acid-producing ability in a medium;
- and
- B) collecting the L-amino acid from the medium,
- wherein the bacterium harbors a mutant pitA gene coding for a phosphate transporter wherein the amino acid residue at position 246 in SEQ ID NO:
- 6 is replaced with an amino acid residue other than a phenylalanine residue.
2 more elements
A DNA coding for a protein selected from the group consisting of:
- (A) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 6 or 26, and
- (B) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 6 or 26, but includes one or more substitutions, deletions, insertions, or additions of one or several amino acid residues, and wherein said protein has phosphate transporter activity;
- wherein the amino acid residue at position 246 in SEQ ID NO:
- 6 or SEQ ID NO:
- 26 is replaced with a serine residue.
4 more elements
A coryneform bacterium harboring a mutant pitA gene coding for a protein selected from the group consisting of:
- (A) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 6 or 26, and
- (B) a protein comprising the amino acid sequence shown in SEQ ID NO:
- 6 or 26, but includes one or more substitutions, deletions, insertions, or additions of one or several amino acid residues, and wherein said protein has phosphate transporter activity;
- wherein the amino acid residue at position 246 in SEQ ID NO:
- 6 or SEQ ID NO:
- 26 is replaced with a serine residue.
4 more elements
Dependencies are inferred from the claim text.