Molecular biology tools for algal engineering
AssigneeLife Technologies Corporation
InventorsFarzad HAERIZADEH, Todd PETERSON, Wen CHEN, Ewa LIS
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Abstract
The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
Claims21
A composition for transformation of an Algal cell with DNA comprising:
- (a) one or more sugars, and
- (b) a biological buffer.
A method for transformation of DNA into an algal cell comprising:
- (a) suspending the algal cells in a buffer,
- (b) adding DNA to be transformed to the algal cell suspension,
- (c) applying an electric pulse to the DNA Algal cell suspension thereby transforming the algal cell with the DNA.
The method of 11, wherein the pH of the buffer is from pH 8 to pH 10.
A kit for the transformation of DNA into an Algal cell comprising:
- (a) a buffer,
- (b) algal cells.
21-27. (canceled)
Dependencies are inferred from the claim text.