Transgenic Plants With Enhanced Traits
AssigneeMonsanto Technology LLC
InventorsMark S. Abad, Monnanda S. Rajani, Tyamagondlu V. Venkatesh, Kammaradi R. Vidya
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Abstract
This disclosure provides plants having enhanced traits such as increased yield, increased nitrogen use efficiency and increased water use efficiency; propagules, progeny and field crops of such plants; and methods of making and using such plants. This disclosure also provides methods of producing seed from such plants, growing such seed and selecting progeny plants with the composition, or with enhanced traits. Also disclosed are plants with altered phenotypes which are useful for screening and selecting events for the desired enhanced trait.
Claims20
A plant comprising a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:
- a) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 23, 24, 25, 26, 27, 28, or 29;
- and
- b) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 23, 24, 25, 26, 27, 28 or 29;
- wherein said plant has at least one enhanced trait as compared to a control plant.
A plant comprising a recombinant DNA molecule comprising a polynucleotide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:
- a) a nucleotide sequence set forth as SEQ ID NO:
- 17, or 20;
- b) a nucleotide sequence that suppresses at least one target gene set forth as SEQ ID NO:
- 18, or 21;
- c) a nucleotide sequence that expresses a RNA that suppresses the expression of a protein having the amino acid sequence of SEQ ID NO:
- 19 or 22;
- d) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 17 or 20;
- e) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 18 or 21;
- and
- f) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 19 or 22;
- wherein said plant has at least one enhanced trait as compared to a control plant.
A method for producing a plant comprising:
- introducing into a plant cell a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:
- a) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 23, 24, 25, 26, 27, 28, or 29;
- and
- b) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 23, 24, 25, 26, 27, 28, or 29;
- and
- growing a plant from said plant cell.
A method for producing a plant comprising:
- introducing into a plant cell a recombinant DNA molecule comprising a nucleotide sequence, wherein said nucleotide sequence suppresses at least one target gene encoding at least one target protein, and wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence set forth as SEQ ID NO:
- 17, or 20;
- b) a nucleotide sequence that suppresses at least one target gene set forth as SEQ ID NO:
- 18, or 21;
- and
- c) a nucleotide sequence that suppresses at least one target gene encoding a target protein set forth as SEQ ID NO:
- 19, or 22;
- and
- growing a plant from said plant cell.
A plant comprising a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:
- a) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:
- 12, or 16;
- and
- b) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 12, or 16;
- and
- wherein said plant has at least one phenotype selected from the group consisting of anthocyanin score, biomass, canopy area, chlorophyll score, plant height, water applied, water content score and water use efficiency that is altered for said plant as compared to a control plant.
A plant comprising a recombinant DNA molecule comprising a nucleotide sequence, wherein said nucleotide sequence suppresses at least one target gene encoding at least one target protein, and wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence set forth as SEQ ID NO:
- 20;
- b) a nucleotide sequence that suppresses at least one target gene set forth as SEQ ID NO:
- 21;
- c) a nucleotide sequence that suppresses at least one target gene encoding a target protein set forth as SEQ ID NO:
- 22;
- d) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 20;
- e) a nucleotide sequence that suppresses at least one target gene with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 21;
- and
- f) a nucleotide sequence that suppresses at least one target gene encoding a target protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
- 22;
- wherein said plant has at least one phenotype selected from the group consisting of anthocyanin score, biomass, canopy area, chlorophyll score, plant height, water applied, water content score and water use efficiency that is altered for said plant as compared to a control plant.
Dependencies are inferred from the claim text.