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US 2015/0307892 A1Published application
Modulation of expression of acyltransferases to modify hydroxycinnamic acid content
AssigneeThe Regents of the University of California
InventorsLaura E. Bartley, Pamela Ronald, Henrik Vibe Scheller
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Abstract
The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.
Claims20
A method of engineering a plant to decrease the ferulic acid content in the plant, the method comprising:
- introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT10, AT7, or AT15 acyltransferase wherein the acyltransferase has at least 70% identity to a sequence selected from SEQ ID NO:2, SEQ ID NO:4, or SEQ ID NO:6, and
- culturing the plant under conditions in which the acyltransferase is expressed.
A method of engineering a plant to increase the ferulic acid content in the plant, the method comprising:
- introducing an expression cassette into the plant, wherein the expression cassette comprises a polynucleotide encoding an AT5 acyltransferase wherein the acyltransferase has at least 70% identity to SEQ ID NO:8, and
- culturing the plant under conditions in which the acyltransferase is expressed.
A plant cell comprising a polynucleotide encoding an AT10, AT15, AT7, or AT5 operably linked to a heterologous promoter;
- or a plant comprising a heterologous polynucleotide encoding an AT10, AT15, AT7, or AT5 protein.
Dependencies are inferred from the claim text.