Double-stranded ribonucleic acid for adjuvants
AssigneeKyowa Hakko Bio Co., Ltd.
InventorsTetsuo NAKANO, Eitora YAMAMURA
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Abstract
The invention provides a double-stranded ribonucleic acid (dsRNA) having a chain length suitable for simultaneously showing low toxicity and high function in the use of an adjuvant and the like, and resisting variation of chain length even when subjected to a heating and cooling treatment, or a salt thereof; an immune potentiator, adjuvant, pharmaceutical product and the like containing the dsRNA and the like; and a production method of such dsRNA and the like. The invention is characterized in that the weight average chain length of two or more single-stranded ribonucleic acids (ssRNAs) constituting the first chain constituting dsRNA is not more than ½ of the weight average chain length of one ssRNA constituting the second chain.
Claims18
A double-stranded ribonucleic acid (dsRNA) having a weight average chain length within the range of 0.1 kilobase pairs (kbp) to 2.0 kbp, or a salt thereof, wherein
- the first chain of said dsRNA consists of two or more single-stranded ribonucleic acids (ssRNAs), and all of two or more ssRNAs constituting said first chain are homopolymers consisting of the same type of ribonucleotide,
- the second chain of said dsRNA consists of one ssRNA, and each of two or more ssRNAs constituting said first chain has a base sequence having complementarity of a level capable of forming a double strand to a partial region of one ssRNA constituting said second chain, and
- the weight average chain length of two or more ssRNAs constituting said first chain is not more than ½ to the weight average chain length of one ssRNA constituting said second chain.
A method of producing double-stranded ribonucleic acid (dsRNA) having a weight average chain length within the range of 0.1 kilobase pairs (kbp) to 2.0 kbp, or a salt thereof, comprising
- (1) a step of preparing ssRNA consisting of the same type of ribonucleotide,
- (2) a step of preparing an ssRNA having a base sequence having complementarity of a level capable of forming a double strand to said (1) ssRNA, and
- (3) a step of annealing said (1) ssRNA and said (2) ssRNA,
- wherein the weight average chain length of ssRNA in said (1) is not more than ½ to that of said (2) and is 0.02-1.0 kilobase (kb).
Dependencies are inferred from the claim text.