Anoikis resistant placental stem cells and uses thereof
AssigneeAnthrogenesis Corporation
InventorsXiaokui Zhang, Stewart Abbot, Vanessa Voskinarian-Berse
Life of this patent
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Abstract
Anoikis resistant placental stem cells (arPSCs) with increased survival in low-attachment environments, and thus can advantageously be used, e.g., in therapies based on their ability to persist for longer durations of time in an unattached state. A method of modifying placental stem cells to make them anoikis resistant, comprising contacting the placental stem cells with an effective amount of modulatory RNA molecules, such that one or more genes associated with anoikis of the placental stem cells is inhibited. Further discloses are genes that are associated with anoikis for modulation.
Claims24
An isolated placental stem cell, wherein said placental stem cell is resistant to anoikis.
An isolated placental stem cell, wherein said placental stem cell expresses at least one anoikis associated gene at a decreased level as compared to the expression of the same anoikis associated gene in an unmodified placental stem cell.
An isolated population of cells comprising anoikis resistant placental stem cells.
A method of producing anoikis resistant placental stem cells comprising contacting the placental stem cells with an effective amount of modulatory RNA molecules, such that said placental stem cells, after having been contacted with said modulatory RNA molecules (i) express at least one anoikis associated gene at a decreased level as compared to the expression of the same anoikis associated gene in an equivalent amount of placental stem cells not contacted with said modulatory RNA molecules and/or (ii) survive in a low-attachment environment for a longer duration of time than an equivalent amount of placental stem cells not contacted with said modulatory RNA molecules.
Dependencies are inferred from the claim text.