Device for capture and lysis of microorganisms from liquids and methods of use thereof

A method for detecting a microbial contaminant in a liquid comprising:

• a) filtering a sample of a liquid through the device of claim 1, wherein microorganisms in the liquid are captured by the filter within the input cavity;
• b) sealing the output end of the device;
• c) dispensing into the input cavity an amount of a lysis solution sufficient to cover the filter;
• d) sealing the input end of the device;
• e) agitating the device, wherein agitating causes the at least one bead to strike the membrane, at least one captured microorganism, an inner surface of the device or a combination thereof, thereby forming a lysate of the captured microorganisms;
• f) recovering the lysate from the device;
• and
• g) detecting the presence or absence of a nucleic acid of at least one of the microorganisms in the recovered lysate, wherein the presence of the nucleic acid indicates a microbial contaminant in the liquid.