Yeast strain improvement method
AssigneesInstitut Curie, Centre National De La Recherche Scientifique, Universite Pierre et Marie Curie (Paris6), Universite Nice Sophia Antipolis, Inserm (Institut National De La Sante Et De La Recherche Medicale)
InventorsALAIN NICOLAS, GIANNI LITI
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Abstract
The present invention relates to a method for improving an industrial yeast strain of industrial interest, particularly a sterile hybrid strain, without resorting to recombinant DNA techniques
Claims23
1-26. (canceled)
A method for improving a yeast strain of industrial interest, comprising:
- a) transferring the yeast from a rich medium to a sporulation medium;
- b) incubating the yeasts in the sporulation medium for a length of time sufficient to induce the formation of Spo11-dependent double-strand breaks;
- c) placing the yeasts in contact with a source of carbon and nitrogen before the reductional chromosome segregation of the first meiotic division in order to obtain the recombinant yeasts;
- d) collecting the recombinant yeasts;
- and
- e) screening or selection of the recombinant yeasts in order to identify those having the desired improvement.
A method for generating a recombinant yeast library from a yeast, comprising:
- a) transferring the yeast from a rich medium to a sporulation medium;
- b) incubating the yeasts in the sporulation medium for a length of time sufficient to induce Spo11-dependent double-strand breaks:
- (c) placing the yeasts in contact with a source of carbon and nitrogen before the reductional chromosome segregation of the first meiotic division in order to obtain recombinant yeasts;
- and
- d) collecting the recombinant yeasts in order to form a recombinant yeast library.
A method for identifying or locating in a yeast the genetic information encoding a characteristic of interest comprising:
- a) transferring the yeast from a rich medium to a sporulation medium;
- b) incubating the yeasts in the sporulation medium for a length of time sufficient to induce Spo11-dependent double-strand breaks;
- c) placing the yeast in contact with a source of carbon and nitrogen before the reductional chromosome segregation of the first meiotic division in order to obtain the recombinant yeasts;
- d) collecting the recombinant yeasts;
- and
- e) analyzing the genotypes and phenotypes of the recombinant yeasts to identify or locate the genetic information encoding the characteristic of interest.
Dependencies are inferred from the claim text.