Acyl-ACP Thioesterase
AssigneeKao Corporation
InventorsTatsuro OZAKI, Hiroyuki OHTA, Koichi HORI
Life of this patent
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Abstract
An acyl-ACP thioesterase consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO: 1; an acyl-ACP thioesterase gene encoding the protein; a transformant having the gene; and a method of producing a lipid using the transformant.
Claims21
-14. (canceled)
A method of modifying a fatty acid composition in a lipid, comprising introducing a gene encoding a protein selected from the following (a) to (c) or a recombinant vector comprising the gene into a host:
- (a) A protein consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO:
- 1;
- (b) A protein consisting of an amino acid sequence having 50% or more identity with the amino acid sequence of the protein (a), and having acyl-ACP thioesterase activity;
- and
- (c) A protein comprising the amino acid sequence of the protein (a) or (b), and having acyl-ACP thioesterase activity.
A method of modifying a fatty acid composition in a lipid, comprising introducing a gene consisting of any one of the following DNAs (d) to (f) or a recombinant vector comprising the gene into a host:
- (d) A DNA consisting of a nucleotide sequence of the 343rd to 825th nucleotides set forth in SEQ ID NO:
- 2;
- (e) A DNA consisting of a nucleotide sequence having 50% or more identity with the nucleotide sequence of the DNA (d), and encoding a protein having acyl-ACP thioesterase activity;
- and
- (f) A DNA comprising the nucleotide sequence of the DNA (d) or (e), and encoding a protein having acyl-ACP thioesterase activity.
A method of enhancing productivity of a lipid, comprising introducing a gene encoding a protein selected from the following (a) to (c) or a recombinant vector comprising the gene into a host:
- (a) A protein consisting of an amino acid sequence of the 115th to 274th amino acids set forth in SEQ ID NO:
- 1;
- (b) A protein consisting of an amino acid sequence having 50% or more identity with the amino acid sequence of the protein (a), and having acyl-ACP thioesterase activity;
- and
- (c) A protein comprising the amino acid sequence of the protein (a) or (b), and having acyl-ACP thioesterase activity.
A method of enhancing productivity of a lipid, comprising introducing a gene consisting of any one of the following DNAs (d) to (f) or a recombinant vector comprising the gene into a host:
- (d) A DNA consisting of a nucleotide sequence of the 343rd to 825th nucleotides set forth in SEQ ID NO:
- 2;
- (e) A DNA consisting of a nucleotide sequence having 50% or more identity with the nucleotide sequence of the DNA (d), and encoding a protein having acyl-ACP thioesterase activity;
- and
- (f) A DNA comprising the nucleotide sequence of the DNA (d) or (e), and encoding a protein having acyl-ACP thioesterase activity.
Dependencies are inferred from the claim text.