Beta-hexosyl-transferases and uses thereof
AssigneeNorth Carolina State University
InventorsJose M. Bruno-Barcena, Suzanne Dagher, Maria Azcarate-Peril
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Abstract
This invention relates generally to the discovery of novel recombinant forms of β-hexosyl-transferases (BHT) and uses thereof to produce galacto-ligosaccharides (GOS) or as food additives.
Claims22
An isolated DNA coding for a recombinant β-hexosyl-transferase (rBHT) protein having the amino acid sequence set forth in SEQ ID NO. 4, 6, 8, 10, 12, 14, 16, 18 or 20.
An enzymatically active recombinant β-hexosyl-transferase (rBHT) protein wherein the protein has the amino acid sequence set forth in SEQ ID NO. 4, 6, 8, 10, 12, 14, 16, 18 or 20.
A method for producing enzymatically active recombinant β-hexosyl-transferase (rBHT) protein in a eukaryotic host cell which comprises transforming the eukaryotic host cell with a plasmid under the control of a suitable promotor wherein the plasmid contains an isolated DNA coding for a rBHT protein having the amino acid sequence set forth in SEQ ID NO. 4, 6, 8, 10, 12, 14, 16, 18 or 20.
A method for producing galacto-oligosaccharides (GOS) which comprises reacting lactose with an enzymatically active recombinant β-hexosyl-transferase (rBHT) protein having the amino acid sequence set forth in SEQ ID NO. 4, 6, 8, 10, 12, 14, 16, 18 or 20 under suitable conditions so as to produce GOS.
A modified lactose-containing foodstuff or food byproduct comprising a recombinant β-hexosyl-transferase (rBHT) protein having the amino acid sequence set forth in SEQ ID NO. 4, 6, 8, 10, 12, 14, 16, 18 or 20.
Dependencies are inferred from the claim text.