Growth factor cocktail to enhance osteogenic differentiation of mesenchymal cells
AssigneesKatholieke Universiteit Leuven, University of Pennsylvania
InventorsChristopher Chen, Frank Luyten, Jeroen Eyckmans, Jan Schrooten, Scott Roberts
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Abstract
The present invention relates to methods and compositions for osteogenic differentiation of human periosteum derived cells, in particular using a growth medium containing a specific combination of growth factors and formulations thereof. The invention also relates to the differentiated cells and cell populations, as well as further products comprising such cells and uses thereof in bone therapy.
Claims30
A method for inducing cells to proliferate and differentiate into cells with a osteogenic phenotype, the method comprising culturing cells in a medium comprising about 2 ng/ml to about 200 ng/ml EGF, about 1 ng/ml to about 100 ng/ml IL6, and about 1 ng/ml to about 100 ng/ml TGFβ1.
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A composition, comprising cells in a culture medium comprising about 2 ng/ml to about 200 ng/ml EGF, about 1 ng/ml to about 100 ng/ml IL6 and about 1 ng/ml to about 100 ng/ml TGFβ1, wherein the cells express a primitive mesenchymal phenotype in the culture medium.
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Dependencies are inferred from the claim text.