Apparatus for Enhanced Recovery of Regenerative cells From Tissue Samples
AssigneeIngeneron, Inc.
InventorsEckhard U. Alt, Ron Stubbers, Michael E. Coleman
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Abstract
This document describes methods and an apparatus for recovery of a cell-enriched matrix and cells (e.g., regenerative cells) from a tissue sample. In some embodiments, at least two rounds of acceleration and deceleration are performed.
Claims16
A cellular composition comprising the combination of (i) a cell-enriched matrix prepared from a sample of body tissue having vasculature and regenerative cells adaptive to differentiate into functional equivalent of host tissue after transplantation therein, by extruding the body tissue sample through an orifice to reduce lipid content and increase regenerative cell content in the sample, followed by centrifugation of the extruded sample at a centrifugal force and duration sufficient to derive therefrom the cell-enriched matrix with regenerative platform therein;
- together with (ii) a regenerative cell population recovered by subjecting such a derived cell-enriched matrix to repetitive cycles of rotational acceleration and deceleration of at least one cycle thereof per minute in a temperature-controlled centrifuge with enzymatic environment to dissociate and extract regenerative cells from the matrix.
Dependencies are inferred from the claim text.