Cell culture method
AssigneeIsopogen Pty Ltd
InventorMarian June STURM
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Abstract
The present invention is directed towards methods for producing cell and tissue compositions suitable for therapeutic applications to a mammal in need of a therapeutic cell or tissue treatment. In particular, the invention is directed towards a method of culturing cells for use in therapy, which method comprises (i) culturing a sample of cells in a first cell culture medium and (ii) prior to harvesting cells for use in therapy culturing the cells in a second cell culture medium.
Claims17
1-51. (canceled)
A method of culturing human mesenchymal stromal cells for use in therapy comprising the steps of:
- (i) providing a sample of said cells and culturing same in a first cell culture medium comprising a first serum for sufficient time to enable the cells to achieve a desired level of expansion;
- and
- (ii) prior to harvesting said cells for use in therapy said first cell culture medium is replaced with a second cell culture medium comprising a second serum selected from human serum or human serum albumin and the cells are further cultured for between about 24 hours and about 36 hours before the cells are harvested for use in cell therapy, wherein the first serum is not human serum or human serum albumin.
A method of culturing human mesenchymal stromal cells for use in cell therapy comprising the steps of:
- (i) culturing mesenchymal stromal cells in a first cell culture medium comprising DMEM supplemented with heat inactivated fetal calf serum (HIFCS) until said cells reach about 70-85% confluence;
- and
- (ii) prior to harvesting cells for use in therapy said first cell culture medium is removed and replaced with a second cell culture medium comprising DMEM phenol red-free and about 2% human serum albumin and the cells are then cultured for a further about 24 hours to about 36 hours.
Dependencies are inferred from the claim text.