Ch3 domain variant pair inducing formation of heterodimer of heavy chain constant region of antibody at high efficiency, method for preparing same, and use thereof

A method for preparing a CH3 domain variant (heterodimeric CH3) pair, which the formation of a heterodimeric Fc is preferred, the method comprising:

• (a) preparing a mutation pair by i) in a first group of CH3 domain selected from the group consisting of Tyr349, Asp356, Glu357, Ser364, Lys370, Lys392, Asp399, Phe405 and Lys409, substituting at least one amino acid positioned in the first group with an amino acid selected from the group consisting of alanine (A), threonine (T), serine (S), valine (V), methionine (M) and glycine (G);
• and ii) substituting at least one amino acid positioned in the first group with an amino acid selected from the group consisting of phenylalanine (F), tyrosine (Y) and tryptophan (W);
• (b) preparing mutation pair by i) in a second group of CH3 domain selected from the group consisting of Gln347, Tyr349, Thr350, Ser354, Lys360, Ser364, Asn390, Thr394, Pro395, Val397 and Ser400, substituting at least one amino acid positioned in the second group with lysine (K) or arginine (R);
• and ii) substituting at least one amino acid positioned in the second group with aspartic acid (D) or glutamic acid (E);
• and
• (c) binding the mutation pairs in Step (a) and (b).