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AssigneeBayer HealthCare LLC
InventorsXiao-Yan ZHAO, Zhuozhi WANG, Jian-Ming GU, Ji-Yun KIM, Maxine BAUZON, John E. MURPHY, Kirk MCLEAN, Fang JIN, Tobias MARQUARDT, Xinquan WANG, Andreas WILMEN
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Provided herein are antibodies, antigen-binding antibody fragments (Fabs), and other protein scaffolds, directed against human activated Protein C (aPC) with minimal binding to its zymogen Protein C (PC). Moreover, these aPC binding proteins could potentially block the anti-coagulant activity of aPC to induce coagulation. Therapeutic uses of these binders are described herein as are methods of panning and screening specific antibodies.
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID Nos:
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID Nos:
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a CDR3 comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a light chain variable region comprising an amino acid sequence of SEQ ID NO:8, wherein said amino acid sequence comprises one or more amino acid modifications.
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a heavy chain variable region comprising an amino acid sequence of SEQ ID NO:18, wherein said amino acid sequence comprises one or more amino acid modifications.
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody comprises a light chain variable region comprising an amino acid sequence of SEQ ID NO:12, wherein said amino acid sequence comprises one or more amino acid modifications.
An isolated monoclonal antibody that binds to an epitope of human activated Protein C (human aPC, SEQ ID NO:3), wherein said epitope comprises residues from a heavy chain of human aPC.
An isolated monoclonal antibody that binds to an epitope of human activated Protein C (human aPC, SEQ ID NO:3), wherein said epitope comprises 5195 of SEQ ID NO:3.
An isolated monoclonal antibody that binds to an epitope of human activated Protein C, wherein said epitope comprises one or more residues selected from the group consisting of D60, K96, S97, T98, T99, E170, V171, M172, 5173, M175, A190, 5195, W215, G216, E217, G218, and G218 of SEQ ID NO:3.
An isolated monoclonal antibody that binds to the active site of activated Protein C.
An isolated monoclonal antibody, wherein said antibody binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein said antibody is a fully human antibody.
An isolated nucleic acid molecule encoding an antibody that binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein the antibody comprises a heavy chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID Nos:
An isolated nucleic acid molecule encoding an antibody that binds to activated Protein C and inhibits anticoagulant activity but has minimal binding to unactivated Protein C, wherein the antibody comprises a light chain variable region comprising an amino acid sequence selected from the group consisting of SEQ ID Nos:
Dependencies are inferred from the claim text.